Mouse VEGFA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse VEGFA in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA Kit
• Detection Range: 15.625 to 1000 pg/ml
• Sensitivity: 9.375 pg/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Mouse VEGFA ELISA Kit (A78962) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse VEGFA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for VEGFA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the VEGFA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-VEGFA Antibody, which binds the captured VEGFA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of VEGFA captured in each well. The concentration of VEGFA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.