Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Reverse Triiodothyronine/Reverse T3
- Description:Rat reverse triiodothyronine/reverse T3 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Reverse Triiodothyronine/Reverse T3 ELISA kit exhibits high specificity and excellent specificity for the detection of rat Reverse Triiodothyronine/Reverse T3. No significant cross-reactivity or interference between Reverse Triiodothyronine/Reverse T3 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:93.75 - 6000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:56.25 pg/ml
- Regulatory Status:RUO
- Cat. No.:76741-274
Specifications
About this item
Rat Reverse Triiodothyronine/Reverse T3 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat Reverse Triiodothyronine/Reverse T3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 93.75 - 6000 pg/ml
- Sensitivity: 56.25 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Reverse Triiodothyronine/Reverse T3 ELISA kit (A80153) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Reverse Triiodothyronine/Reverse T3 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Reverse Triiodothyronine/Reverse T3 antigen. During the incubation, Reverse Triiodothyronine/Reverse T3 present in the samples or standards competes with the fixed amount of immobilized Reverse Triiodothyronine/Reverse T3 for binding sites on the Biotinylated Anti-Reverse Triiodothyronine/Reverse T3 Antibody. The more Reverse Triiodothyronine/Reverse T3 present in a sample or standard, the less Biotinylated Anti-Reverse Triiodothyronine/Reverse T3 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Reverse Triiodothyronine/Reverse T3 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Reverse Triiodothyronine/Reverse T3 present in each sample or standard. The concentration of Reverse Triiodothyronine/Reverse T3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.