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Human BDNF ELISA Kit
  76741-254
 :  ANTIBODIES.COM LLC
 :  
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Human BDNF ELISA Kit
  76741-254
 :  ANTIBODIES.COM LLC
 :  A77748-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    BDNF
  • Description:
    Human BDNF ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human BDNF ELISA Kit exhibits high specificity and excellent specificity for the detection of human BDNF. No significant cross-reactivity or interference between BDNF and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    31.25 - 2000 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    18.75 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76741-254

 

 

Human BDNF ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BDNF in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 31.25 to 2000 pg/ml
  • Sensitivity: 18.75 pg/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human BDNF ELISA Kit (A77748) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human BDNF in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for BDNF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BDNF present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-BDNF Antibody, which binds the captured BDNF present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of BDNF captured in each well. The concentration of BDNF can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.