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Human Nociceptin ELISA Kit
Human Nociceptin ELISA Kit
  76740-704
 :  ANTIBODIES.COM LLC
 :  
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Human Nociceptin ELISA Kit
  76740-704
 :  ANTIBODIES.COM LLC
 :  A75678-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    Nociceptin
  • Description:
    Human nociceptin ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human Nociceptin ELISA kit exhibits high specificity and excellent specificity for the detection of human Nociceptin. No significant cross-reactivity or interference between Nociceptin and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    4.688 - 300 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    2.813 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76740-704

 

 

Human Nociceptin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Nociceptin in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 4.688 to 300 pg/ml
  • Sensitivity: 2.813 pg/ml
  • Sample type: Serum. plasma. tissue homogenates. and other biological fluids
  • Assay precision: Intra assay: CV <8%. Inter assay: CV <10%

Human Nociceptin ELISA kit (A75678) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Nociceptin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Nociceptin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Nociceptin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Nociceptin Antibody, which binds the captured Nociceptin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Nociceptin captured in each well. The concentration of Nociceptin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.