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Human GARS ELISA Kit
Human GARS ELISA Kit
  76738-144
 :  ANTIBODIES.COM LLC
 :  
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Human GARS ELISA Kit
  76738-144
 :  ANTIBODIES.COM LLC
 :  A76596-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    GARS
  • Description:
    Human GARS ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human GARS ELISA Kit exhibits high specificity and excellent specificity for the detection of human GARS. No significant cross-reactivity or interference between GARS and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    0.313 - 20 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    0.188 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76738-144

 

 

Human GARS ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GARS in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready To Use ELISA Kit
  • Detection Range: 0.313 to 20 ng/ml
  • Sensitivity: 0.188 ng/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human GARS ELISA Kit (A76596) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human GARS in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GARS has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GARS present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GARS Antibody, which binds the captured GARS present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GARS captured in each well. The concentration of GARS can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.