Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:HLA DRB5
- Description:Human HLA DRB5 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human HLA DRB5 ELISA Kit exhibits high specificity and excellent specificity for the detection of human HLA DRB5. No significant cross-reactivity or interference between HLA DRB5 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:1.563 - 100 ng/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:0.938 ng/ml
- Regulatory Status:RUO
- Cat. No.:76733-384
Specifications
About this item
Human HLA DRB5 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human HLA DRB5 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 1.563 to 100 ng/ml
- Sensitivity: 0.938 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human HLA DRB5 ELISA Kit (A74409) employs the competitive enzyme immunoassay technique for the quantitative measurement of human HLA DRB5 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with HLA DRB5 antigen. During the incubation, HLA DRB5 present in the samples or standards competes with the fixed amount of immobilized HLA DRB5 for binding sites on the Biotinylated Anti-HLA DRB5 Antibody. The more HLA DRB5 present in a sample or standard, the less Biotinylated Anti-HLA DRB5 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-HLA DRB5 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of HLA DRB5 present in each sample or standard. The concentration of HLA DRB5 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.