Mouse Insulin receptor beta ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Insulin receptor beta in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA Kit
• Detection Range: 0.938 to 60 ng/ml
• Sensitivity: 0.563 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Mouse Insulin Receptor beta ELISA Kit (A74871) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Insulin Receptor beta in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Insulin Receptor beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Insulin Receptor beta present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Insulin Receptor beta Antibody, which binds the captured Insulin Receptor beta present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Insulin Receptor beta captured in each well. The concentration of Insulin Receptor beta can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.