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Specifications
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Rat
- Target protein:HSPG
- Description:Rat HSPG ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Heparan Sulphate Proteoglycan/HSPG ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Heparan Sulphate Proteoglycan/HSPG. No significant cross-reactivity or interference between Heparan Sulphate Proteoglycan/HSPG and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.313 - 20 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.188 ng/ml
- Regulatory Status:RUO
- Cat. No.:76732-344
Specifications
About this item
Rat Heparan Sulphate Proteoglycan/HSPG ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Heparan Sulphate Proteoglycan/HSPG in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 0.313 to 20 ng/ml
- Sensitivity: 0.188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Heparan Sulphate Proteoglycan / HSPG ELISA Kit (A79445) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Heparan Sulphate Proteoglycan / HSPG in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Heparan Sulphate Proteoglycan / HSPG has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Heparan Sulphate Proteoglycan / HSPG present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Heparan Sulphate Proteoglycan / HSPG Antibody, which binds the captured Heparan Sulphate Proteoglycan / HSPG present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Heparan Sulphate Proteoglycan / HSPG captured in each well. The concentration of Heparan Sulphate Proteoglycan / HSPG can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.