- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Liver Arginase
- Description:Rat Liver Arginase ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Liver Arginase ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Liver Arginase. No significant cross-reactivity or interference between Liver Arginase and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:2.5 - 160 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:1.5 ng/ml
- Regulatory Status:RUO
- Cat. No.:76747-208
Rat Liver Arginase ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Liver Arginase in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 2.5 to 160 ng/ml
- Sensitivity: 1.5 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Liver Arginase ELISA Kit (A79898) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Liver Arginase in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Liver Arginase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Liver Arginase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Liver Arginase Antibody, which binds the captured Liver Arginase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Liver Arginase captured in each well. The concentration of Liver Arginase can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.