- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:PKC epsilon
- Description:Mouse PKC epsilon ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse PKC epsilon ELISA kit exhibits high specificity and excellent specificity for the detection of mouse PKC epsilon. No significant cross-reactivity or interference between PKC epsilon and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.313 - 20 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.188 ng/ml
- Regulatory Status:RUO
- Cat. No.:76747-108
Mouse PKC epsilon ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse PKC epsilon in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0.313 - 20 ng/ml
- Sensitivity: 0.188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse PKC epsilon ELISA kit (A77146) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse PKC epsilon in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PKC epsilon has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PKC epsilon present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PKC epsilon Antibody, which binds the captured PKC epsilon present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PKC epsilon captured in each well. The concentration of PKC epsilon can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.