Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:EDA
- Description:Human EDA ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human EDA ELISA Kit exhibits high specificity and excellent specificity for the detection of human EDA. No significant cross-reactivity or interference between EDA and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:78.125 - 5000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:46.875 pg/ml
- Regulatory Status:RUO
- Cat. No.:76746-628
Specifications
About this item
Human EDA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human EDA in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 78.125 to 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human EDA ELISA Kit (A79280) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EDA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for EDA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EDA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-EDA Antibody, which binds the captured EDA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of EDA captured in each well. The concentration of EDA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.