- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Aprataxin
- Description:Human aprataxin ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human Aprataxin ELISA Kit exhibits high specificity and excellent specificity for the detection of human Aprataxin. No significant cross-reactivity or interference between Aprataxin and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:31.25 - 2000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:18.75 pg/ml
- Regulatory Status:RUO
- Cat. No.:76745-788
Human Aprataxin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Aprataxin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 31.25 to 2000 pg/ml
- Sensitivity: 18.75 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Aprataxin ELISA kit (A87384) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Aprataxin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Aprataxin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aprataxin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Aprataxin Antibody, which binds the captured Aprataxin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Aprataxin captured in each well. The concentration of Aprataxin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.