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- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Monkey
- Target protein:Tissue Plasminogen Activator
- Description:Monkey tissue plasminogen activator ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross Reactivity:Monkey Tissue Plasminogen Activator ELISA Kit exhibits high specificity and excellent specificity for the detection of monkey Tissue Plasminogen Activator. No significant cross-reactivity or interference between Tissue Plasminogen Activator and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.625 - 40 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.375 ng/ml
- Regulatory Status:RUO
- Cat. No.:76744-828
Monkey Tissue Plasminogen Activator ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey Tissue Plasminogen Activator in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Monkey Tissue Plasminogen Activator ELISA kit (A303629) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Tissue Plasminogen Activator in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Tissue Plasminogen Activator has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Tissue Plasminogen Activator present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Tissue Plasminogen Activator Antibody, which binds the captured Tissue Plasminogen Activator present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Tissue Plasminogen Activator captured in each well. The concentration of Tissue Plasminogen Activator can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.