- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Apolipoprotein CIII
- Description:Human apolipoprotein CIII ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human Apolipoprotein CIII ELISA Kit exhibits high specificity and excellent specificity for the detection of human Apolipoprotein CIII. No significant cross-reactivity or interference between Apolipoprotein CIII and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:7.813 - 500 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:4.688 ng/ml
- Regulatory Status:RUO
- Cat. No.:76743-318
Human Apolipoprotein CIII ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Apolipoprotein CIII in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 7.813 to 500 ng/ml
- Sensitivity: 4.688 ng/ml
- Sample type: Serum. plasma. tissue homogenates. and other biological fluids
- Assay precision: Intra assay: CV <8%. Inter assay: CV <10%
Human Apolipoprotein CIII ELISA Kit (A76159) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Apolipoprotein CIII in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Apolipoprotein CIII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Apolipoprotein CIII present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Apolipoprotein CIII Antibody, which binds the captured Apolipoprotein CIII present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Apolipoprotein CIII captured in each well. The concentration of Apolipoprotein CIII can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.