Specifications
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Complement C4
- Description:Rat Complement Component 4 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Complement C4 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Complement C4. No significant cross-reactivity or interference between Complement C4 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.781 - 50 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.469 ng/ml
- Regulatory Status:RUO
- Cat. No.:76743-298
Specifications
About this item
Rat Complement C4 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Complement C4 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 0.781 to 50 ng/ml
- Sensitivity: 0.469 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Complement C4 ELISA kit (A80258) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Complement C4 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Complement C4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C4 Antibody, which binds the captured Complement C4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C4 captured in each well. The concentration of Complement C4 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.