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Rat Pro-ANP ELISA Kit
Rat Pro-ANP ELISA Kit
  76743-018
 :  ANTIBODIES.COM LLC
 :  
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Rat Pro-ANP ELISA Kit
  76743-018
 :  ANTIBODIES.COM LLC
 :  A75760-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Rat
  • Target protein:
    Pro-ANP
  • Description:
    Rat Pro-ANP ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Rat Pro-ANP ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Pro-ANP. No significant cross-reactivity or interference between Pro-ANP and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    15.625 - 1000 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    9.375 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76743-018

 

 

Rat Pro-ANP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Pro-ANP in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 15.625 to 1000 pg/ml
  • Sensitivity: 9.375 pg/ml
  • Sample type: Serum. plasma. tissue homogenates. and other biological fluids
  • Assay precision: Intra assay: CV <8%. Inter assay: CV <10%

Rat Pro-ANP ELISA Kit (A75760) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Pro-ANP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Pro-ANP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Pro-ANP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Pro-ANP Antibody, which binds the captured Pro-ANP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Pro-ANP captured in each well. The concentration of Pro-ANP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.