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Monkey Clusterin ELISA Kit
Monkey Clusterin ELISA Kit
  76742-468
 :  ANTIBODIES.COM LLC
 :  
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Monkey Clusterin ELISA Kit
  76742-468
 :  ANTIBODIES.COM LLC
 :  A303632-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Monkey
  • Target protein:
    Clusterin
  • Description:
    Monkey clusterin ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates, and other biological fluids
  • Cross Reactivity:
    Monkey Clusterin ELISA Kit exhibits high specificity and excellent specificity for the detection of monkey Clusterin. No significant cross-reactivity or interference between Clusterin and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    3.13 - 200 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    1.875 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76742-468

 

 

Monkey Clusterin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of monkey Clusterin in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Detection Range: 3.13 to 200 ng/ml
  • Sensitivity: 1.875 ng/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%

Monkey Clusterin ELISA kit (A303632) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Clusterin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Clusterin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Clusterin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-Clusterin antibody, which binds the captured Clusterin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Clusterin captured in each well. The concentration of Clusterin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.