Specifications

Assay duration:
Multiple steps
Assay Type (ELISA with LOV):
Sandwich
Format:
Pre-coated
Primary antibody reactivity:
Other
Target protein:
C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP
Description:
Porcine C-terminal propeptide of collagen alpha-1(I) chain/PICP ELISA kit
Size:
96 tests
Sample Type:
Serum, plasma, tissue homogenates, and other biological fluids
Cross Reactivity:
Porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP ELISA Kit exhibits high specificity and excellent specificity for the detection of porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP. No significant cross-reactivity or interference between C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP and analogues was observed.
Detection Method:
Colorimetric
Time to Results:
4 h 30 min
Assay Principle:
Quantitative
Shelf Life:
Store for 6 months at 4 °C
Detection Range:
0.156 - 10 ng/ml
Storage Temperature:
4 °C
Sample Volume:
100 μl
Sensitivity:
0.094 ng/ml
Regulatory Status:
RUO
Cat. No.:
76742-438

Specifications

About this item

Porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP in serum, plasma, tissue homogenates, and other biological fluids.

Ready-to-use ELISA kit
Detection Range: 0.156 - 10 ng/ml
Sensitivity: 0.094 ng/ml
Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP ELISA kit (A303672) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP antibody, which binds the captured C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP captured in each well. The concentration of C-terminal Propeptide of Collagen alpha-1(I) Chain/PICP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.