Specifications
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:alpha Synuclein
- Description:Rat SNCa ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat alpha Synuclein ELISA Kit exhibits high specificity and excellent specificity for the detection of rat alpha Synuclein. No significant cross-reactivity or interference between alpha Synuclein and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76741-788
Specifications
About this item
Rat alpha Synuclein ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat alpha Synuclein in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 15.625 to 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat alpha Synuclein ELISA kit (A80161) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat alpha Synuclein in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for alpha Synuclein has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the alpha Synuclein present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-alpha Synuclein Antibody, which binds the captured alpha Synuclein present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of alpha Synuclein captured in each well. The concentration of alpha Synuclein can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.