Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Bcl-XL
- Description:Rat BCL-XL ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross Reactivity:Rat Bcl-XL ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Bcl-XL. No significant cross-reactivity or interference between Bcl-XL and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76741-188
Specifications
About this item
Rat Bcl-XL ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Bcl-XL in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Rat Bcl-XL ELISA kit (A303765) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Bcl-XL in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Bcl-XL has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Bcl-XL present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-Bcl-XL antibody, which binds the captured Bcl-XL present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Bcl-XL captured in each well. The concentration of Bcl-XL can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.