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Human HEXB ELISA Kit
Human HEXB ELISA Kit
  76738-708
 :  ANTIBODIES.COM LLC
 :  
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Human HEXB ELISA Kit
  76738-708
 :  ANTIBODIES.COM LLC
 :  A78251-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    HEXB
  • Description:
    Human HEXB ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human HEXB ELISA Kit exhibits high specificity and excellent specificity for the detection of human HEXB. No significant cross-reactivity or interference between HEXB and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    1.563 - 100 IU/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    0.938 IU/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76738-708

 

 

Human HEXB ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HEXB in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 1.563 to 100 IU/ml
  • Sensitivity: 0.938 IU/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human HEXB ELISA Kit (A78251) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human HEXB in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HEXB has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HEXB present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HEXB Antibody, which binds the captured HEXB present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HEXB captured in each well. The concentration of HEXB can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.