Human PKA beta (catalytic subunit) ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PKA beta (catalytic subunit) in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 78.125 to 5000 pg/ml
• Sensitivity: 46.875 pg/ml
• Sample type: Serum. plasma. tissue homogenates. and other biological fluids
• Assay precision: Intra assay: CV <8%. Inter assay: CV <10%

Human PKA beta (catalytic subunit) ELISA kit (A75753) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PKA beta (catalytic subunit) in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PKA beta (catalytic subunit) has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PKA beta (catalytic subunit) present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PKA beta (catalytic subunit) Antibody, which binds the captured PKA beta (catalytic subunit) present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PKA beta (catalytic subunit) captured in each well. The concentration of PKA beta (catalytic subunit) can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.