Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:beta Endorphin
- Description:Rat beta endorphin ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat beta Endorphin ELISA Kit exhibits high specificity and excellent specificity for the detection of rat beta Endorphin. No significant cross-reactivity or interference between beta Endorphin and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76738-418
Specifications
About this item
Rat beta Endorphin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat beta Endorphin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 15.625 to 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat beta Endorphin ELISA kit (A79804) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat beta Endorphin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with beta Endorphin antigen. During the incubation, beta Endorphin present in the samples or standards competes with the fixed amount of immobilized beta Endorphin for binding sites on the Biotinylated Anti-beta Endorphin Antibody. The more beta Endorphin present in a sample or standard, the less Biotinylated Anti-beta Endorphin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-beta Endorphin Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of beta Endorphin present in each sample or standard. The concentration of beta Endorphin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.