Human alpha Fodrin/alpha II Spectrin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human alpha Fodrin/alpha II Spectrin in serum, plasma, tissue homogenates, and other biological fluids.

• Ready To Use ELISA Kit
• Detection Range: 15.625 to 1000 pg/ml
• Sensitivity: 9.375 pg/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human alpha Fodrin / alpha II Spectrin ELISA Kit (A75026) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human alpha Fodrin / alpha II Spectrin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for alpha Fodrin / alpha II Spectrin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the alpha Fodrin / alpha II Spectrin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-alpha Fodrin / alpha II Spectrin Antibody, which binds the captured alpha Fodrin / alpha II Spectrin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of alpha Fodrin / alpha II Spectrin captured in each well. The concentration of alpha Fodrin / alpha II Spectrin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.