Porcine C-telopeptide of Type II Collagen/CTXII ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine C-telopeptide of Type II Collagen/CTXII in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Detection Range: 15.625 - 1000 pg/ml
• Sensitivity: 9.375 pg/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Porcine C-telopeptide of Type II Collagen/CTXII ELISA kit (A303667) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine C-telopeptide of Type II Collagen/CTXII in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for C-telopeptide of Type II Collagen/CTXII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the C-telopeptide of Type II Collagen/CTXII present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-C-telopeptide of Type II Collagen/CTXII antibody, which binds the captured C-telopeptide of Type II Collagen/CTXII present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of C-telopeptide of Type II Collagen/CTXII captured in each well. The concentration of C-telopeptide of Type II Collagen/CTXII can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.