Mouse High Molecular Weight (HMW) Adiponectin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse High Molecular Weight (HMW) Adiponectin in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Detection range: 15.625 to 1000 pg/ml
• Sensitivity: 9.375 pg/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse High Molecular Weight (HMW) Adiponectin ELISA kit (A303486) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse High Molecular Weight (HMW) Adiponectin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for High Molecular Weight (HMW) Adiponectin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the High Molecular Weight (HMW) Adiponectin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-High Molecular Weight (HMW) Adiponectin antibody, which binds the captured High Molecular Weight (HMW) Adiponectin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of High Molecular Weight (HMW) Adiponectin captured in each well. The concentration of High Molecular Weight (HMW) Adiponectin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.