- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:AVPR1A/V1aR
- Description:Rat AVPR1A/V1aR ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat AVPR1A/V1aR ELISA Kit exhibits high specificity and excellent specificity for the detection of rat AVPR1A/V1aR. No significant cross-reactivity or interference between AVPR1A/V1aR and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:46.875 - 3000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:28.125 pg/ml
- Regulatory Status:RUO
- Cat. No.:76730-318
Rat AVPR1A/V1aR ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat AVPR1A/V1aR in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 46.875 to 3000 pg/ml
- Sensitivity: 28.125 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat AVPR1A / V1aR ELISA Kit (A77727) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat AVPR1A / V1aR in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for AVPR1A / V1aR has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the AVPR1A / V1aR present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-AVPR1A / V1aR Antibody, which binds the captured AVPR1A / V1aR present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of AVPR1A / V1aR captured in each well. The concentration of AVPR1A / V1aR can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.