Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Salusin alpha
- Description:Human salusin alpha ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human Salusin alpha ELISA Kit exhibits high specificity and excellent specificity for the detection of human Salusin alpha. No significant cross-reactivity or interference between Salusin alpha and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:78.125 - 5000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:46.875 pg/ml
- Regulatory Status:RUO
- Cat. No.:76745-850
Specifications
About this item
Human Salusin alpha ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Salusin alpha in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 78.125 to 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Salusin alpha ELISA Kit (A74121) employs the competitive enzyme immunoassay technique for the quantitative measurement of human Salusin alpha in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Salusin alpha antigen. During the incubation, Salusin alpha present in the samples or standards competes with the fixed amount of immobilized Salusin alpha for binding sites on the Biotinylated Anti-Salusin alpha Antibody. The more Salusin alpha present in a sample or standard, the less Biotinylated Anti-Salusin alpha Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Salusin alpha Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Salusin alpha present in each sample or standard. The concentration of Salusin alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.