Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:GRP78 BiP
- Description:Mouse GRP78 BiP ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse GRP78 BiP ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse GRP78 BiP. No significant cross-reactivity or interference between GRP78 BiP and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.469 - 30 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.281 ng/ml
- Regulatory Status:RUO
- Cat. No.:76744-300
Specifications
About this item
Mouse GRP78 BiP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GRP78 BiP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 0.469 to 30 ng/ml
- Sensitivity: 0.281 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse GRP78 BiP ELISA Kit (A77554) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse GRP78 BiP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GRP78 BiP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GRP78 BiP present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GRP78 BiP Antibody, which binds the captured GRP78 BiP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GRP78 BiP captured in each well. The concentration of GRP78 BiP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.