- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:UCN2/SRP
- Description:Mouse UCN2/SRP ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse UCN2/SRP ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse UCN2/SRP. No significant cross-reactivity or interference between UCN2/SRP and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:78.125 - 5000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:46.875 pg/ml
- Regulatory Status:RUO
- Cat. No.:76744-050
Mouse UCN2/SRP ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse UCN2/SRP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 78.125 to 5000 pg/ml
- Sensitivity: 46.875 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse UCN2/SRP ELISA kit (A78948) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse UCN2/SRP in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with UCN2/SRP antigen. During the incubation, UCN2/SRP present in the samples or standards competes with the fixed amount of immobilized UCN2/SRP for binding sites on the Biotinylated Anti-UCN2/SRP Antibody. The more UCN2/SRP present in a sample or standard, the less Biotinylated Anti-UCN2/SRP Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-UCN2/SRP Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of UCN2/SRP present in each sample or standard. The concentration of UCN2/SRP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.