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Porcine CXCL16 ELISA Kit
Porcine CXCL16 ELISA Kit
  76743-840
 :  ANTIBODIES.COM LLC
 :  
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Porcine CXCL16 ELISA Kit
  76743-840
 :  ANTIBODIES.COM LLC
 :  A74186-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    CXCL16
  • Description:
    Porcine CXCL16 ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Porcine CXCL16 ELISA Kit exhibits high specificity and excellent specificity for the detection of porcine CXCL16. No significant cross-reactivity or interference between CXCL16 and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    62.5 - 4000 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    37.5 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76743-840

 

 

Porcine CXCL16 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine CXCL16 in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready To Use ELISA Kit
  • Detection Range: 62.5 to 4000 pg/ml
  • Sensitivity: 37.5 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Porcine CXCL16 ELISA Kit (A74186) employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine CXCL16 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CXCL16 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CXCL16 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CXCL16 Antibody, which binds the captured CXCL16 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CXCL16 captured in each well. The concentration of CXCL16 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.