Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:ACADM/MCAD
- Description:Rat ACADM/MCAD ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat ACADM/MCAD ELISA Kit exhibits high specificity and excellent specificity for the detection of rat ACADM/MCAD. No significant cross-reactivity or interference between ACADM/MCAD and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.781 - 50 ng/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:0.469 ng/ml
- Regulatory Status:RUO
- Cat. No.:76740-550
Specifications
About this item
Rat ACADM/MCAD ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat ACADM/MCAD in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 0.781 to 50 ng/ml
- Sensitivity: 0.469 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat ACADM/MCAD ELISA kit (A79070) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat ACADM/MCAD in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with ACADM/MCAD antigen. During the incubation, ACADM/MCAD present in the samples or standards competes with the fixed amount of immobilized ACADM/MCAD for binding sites on the Biotinylated Anti-ACADM/MCAD Antibody. The more ACADM/MCAD present in a sample or standard, the less Biotinylated Anti-ACADM/MCAD Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-ACADM/MCAD Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of ACADM/MCAD present in each sample or standard. The concentration of ACADM/MCAD can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.