Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rabbit
- Target protein:IL-1RA
- Description:Rabbit IL-1RA ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rabbit IL-1RA ELISA Kit exhibits high specificity and excellent specificity for the detection of rabbit IL-1RA. No significant cross-reactivity or interference between IL-1RA and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:31.25 - 2000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:18.75 pg/ml
- Regulatory Status:RUO
- Cat. No.:76739-180
Specifications
About this item
Rabbit IL-1RA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rabbit IL-1RA in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 31.25 to 2000 pg/ml
- Sensitivity: 18.75 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rabbit IL-1RA ELISA Kit (A74212) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rabbit IL-1RA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for IL-1RA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-1RA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-1RA Antibody, which binds the captured IL-1RA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-1RA captured in each well. The concentration of IL-1RA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.