Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Antidiuretic hormone
- Description:Sheep antidiuretic hormone ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Sheep Antidiuretic Hormone ELISA Kit exhibits high specificity and excellent specificity for the detection of sheep Antidiuretic Hormone. No significant cross-reactivity or interference between Antidiuretic Hormone and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76738-990
Specifications
About this item
Sheep Antidiuretic Hormone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of sheep Antidiuretic Hormone in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 15.625 to 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Sheep Antidiuretic Hormone ELISA kit (A87523) employs the competitive enzyme immunoassay technique for the quantitative measurement of sheep Antidiuretic Hormone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Antidiuretic Hormone antigen. During the incubation, Antidiuretic Hormone present in the samples or standards competes with the fixed amount of immobilized Antidiuretic Hormone for binding sites on the Biotinylated Anti-Antidiuretic Hormone Antibody. The more Antidiuretic Hormone present in a sample or standard, the less Biotinylated Anti-Antidiuretic Hormone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Antidiuretic Hormone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Antidiuretic Hormone present in each sample or standard. The concentration of Antidiuretic Hormone can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.