Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Glutathione Reductase
- Description:Rat glutathione reductase ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Glutathione Reductase ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Glutathione Reductase. No significant cross-reactivity or interference between Glutathione Reductase and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.313 - 20 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.188 ng/ml
- Regulatory Status:RUO
- Cat. No.:76737-900
Specifications
About this item
Rat Glutathione Reductase ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Glutathione Reductase in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA kit
- Detection Range: 0.313 to 20 ng/ml
- Sensitivity: 0.188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rat Glutathione Reductase ELISA kit (A80314) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Glutathione Reductase in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Glutathione Reductase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Glutathione Reductase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Glutathione Reductase Antibody, which binds the captured Glutathione Reductase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Glutathione Reductase captured in each well. The concentration of Glutathione Reductase can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.