- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Leptin
- Description:Rat leptin ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rat Leptin ELISA kit exhibits high specificity and excellent specificity for the detection of rat Leptin. No significant cross-reactivity or interference between Leptin and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.156 - 10 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.094 ng/ml
- Regulatory Status:RUO
- Cat. No.:76737-220
Rat Leptin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Leptin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0.156 - 10 ng/ml
- Sensitivity: 0.094 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Leptin ELISA kit (A80327) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Leptin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Leptin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Leptin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Leptin Antibody, which binds the captured Leptin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Leptin captured in each well. The concentration of Leptin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.