Human Casein Kinase 1 delta/CSNK1D ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Casein Kinase 1 delta/CSNK1D in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Detection Range: 0.156 - 10 ng/ml
• Sensitivity: 0.094 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human Casein Kinase 1 delta/CSNK1D ELISA kit (A247290) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Casein Kinase 1 delta/CSNK1D in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Casein Kinase 1 delta/CSNK1D has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Casein Kinase 1 delta/CSNK1D present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-Casein Kinase 1 delta/CSNK1D antibody, which binds the captured Casein Kinase 1 delta/CSNK1D present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Casein Kinase 1 delta/CSNK1D captured in each well. The concentration of Casein Kinase 1 delta/CSNK1D can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.