Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:HLA-B27
- Description:Human HLA-B27 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human HLA-B27 ELISA Kit exhibits high specificity and excellent specificity for the detection of human HLA-B27. No significant cross-reactivity or interference between HLA-B27 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.313 - 20 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.188 ng/ml
- Regulatory Status:RUO
- Cat. No.:76733-240
Specifications
About this item
Human HLA-B27 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HLA-B27 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 0.313 to 20 ng/ml
- Sensitivity: 0.188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human HLA-B27 ELISA Kit (A74816) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human HLA-B27 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HLA-B27 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HLA-B27 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HLA-B27 Antibody, which binds the captured HLA-B27 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HLA-B27 captured in each well. The concentration of HLA-B27 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.