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Sheep E-Selectin/CD62E ELISA Kit
  76732-890
 :  ANTIBODIES.COM LLC
 :  
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Sheep E-Selectin/CD62E ELISA Kit
  76732-890
 :  ANTIBODIES.COM LLC
 :  A303867-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    E-Selectin/CD62E
  • Description:
    Sheep E-Selectin/CD62E ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates, and other biological fluids
  • Cross Reactivity:
    Sheep E-Selectin/CD62E ELISA Kit exhibits high specificity and excellent specificity for the detection of sheep E-Selectin/CD62E. No significant cross-reactivity or interference between E-Selectin/CD62E and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    0.313 - 20 ng/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    0.188 ng/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76732-890

 

 

Sheep E-Selectin/CD62E ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep E-Selectin/CD62E in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Detection Range: 0.313 to 20 ng/ml
  • Sensitivity: 0.188 ng/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%

Sheep E-Selectin/CD62E ELISA kit (A303867) employs the sandwich enzyme immunoassay technique for the quantitative measurement of sheep E-Selectin/CD62E in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for E-Selectin/CD62E has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the E-Selectin/CD62E present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-E-Selectin/CD62E antibody, which binds the captured E-Selectin/CD62E present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of E-Selectin/CD62E captured in each well. The concentration of E-Selectin/CD62E can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.