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Porcine Corticotropin Releasing Hormone ELISA Kit
Porcine Corticotropin Releasing Hormone ELISA Kit
  76732-760
 :  ANTIBODIES.COM LLC
 :  
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Porcine Corticotropin Releasing Hormone ELISA Kit
  76732-760
 :  ANTIBODIES.COM LLC
 :  A75327-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type (ELISA with LOV):
    Competitive
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Other
  • Target protein:
    Corticotropin Releasing Hormone
  • Description:
    Porcine corticotropin releasing hormone ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Porcine Corticotropin Releasing Hormone ELISA kit exhibits high specificity and excellent specificity for the detection of porcine Corticotropin Releasing Hormone. No significant cross-reactivity or interference between Corticotropin Releasing Hormone and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    1.563 - 100 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    50 μl
  • Sensitivity:
    0.938 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76732-760

 

 

Porcine Corticotropin Releasing Hormone ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine Corticotropin Releasing Hormone in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-To-Use ELISA Kit
  • Detection Range: 1.563 - 100 pg/ml
  • Sensitivity: 0.938 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Porcine Corticotropin Releasing Hormone ELISA kit (A75327) employs the competitive enzyme immunoassay technique for the quantitative measurement of porcine Corticotropin Releasing Hormone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Corticotropin Releasing Hormone antigen. During the incubation, Corticotropin Releasing Hormone present in the samples or standards competes with the fixed amount of immobilized Corticotropin Releasing Hormone for binding sites on the Biotinylated Anti-Corticotropin Releasing Hormone Antibody. The more Corticotropin Releasing Hormone present in a sample or standard, the less Biotinylated Anti-Corticotropin Releasing Hormone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Corticotropin Releasing Hormone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Corticotropin Releasing Hormone present in each sample or standard. The concentration of Corticotropin Releasing Hormone can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.