Specifications
- Assay Type (ELISA with LOV):Competitive
- Format:Pre-coated
- Primary antibody reactivity:Rabbit
- Target protein:PTH
- Description:Rabbit PTH ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Rabbit Parathyroid Hormone ELISA Kit exhibits high specificity and excellent specificity for the detection of rabbit Parathyroid Hormone. No significant cross-reactivity or interference between Parathyroid Hormone and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:50 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76732-120
Specifications
About this item
Rabbit Parathyroid Hormone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rabbit Parathyroid Hormone in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 15.625 to 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Rabbit Parathyroid Hormone ELISA Kit (A87464) employs the competitive enzyme immunoassay technique for the quantitative measurement of rabbit Parathyroid Hormone in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Parathyroid Hormone antigen. During the incubation, Parathyroid Hormone present in the samples or standards competes with the fixed amount of immobilized Parathyroid Hormone for binding sites on the Biotinylated Anti-Parathyroid Hormone Antibody. The more Parathyroid Hormone present in a sample or standard, the less Biotinylated Anti-Parathyroid Hormone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Parathyroid Hormone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Parathyroid Hormone present in each sample or standard. The concentration of Parathyroid Hormone can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.