- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:TRAP/CD40L
- Description:Mouse TRAP/CD40L ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Mouse TRAP/CD40L ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse TRAP/CD40L. No significant cross-reactivity or interference between TRAP/CD40L and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:1.563 - 100 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.938 ng/ml
- Regulatory Status:RUO
- Cat. No.:76730-960
Mouse TRAP/CD40L ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse TRAP/CD40L in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 1.563 to 100 ng/ml
- Sensitivity: 0.938 ng/ml
- Sample type: Serum. plasma. tissue homogenates. and other biological fluids
- Assay precision: Intra assay: CV <8%. Inter assay: CV <10%
Mouse TRAP / CD40L ELISA Kit (A76283) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse TRAP / CD40L in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for TRAP / CD40L has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TRAP / CD40L present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TRAP / CD40L Antibody, which binds the captured TRAP / CD40L present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TRAP / CD40L captured in each well. The concentration of TRAP / CD40L can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.