Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.

• Uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand
• For the purification of 6x His proteins
• High capacity: >50 mg/ml
• Ligand density: 20 to 40 μM CO₂⁺ /ml resin
• Bead Structure: 6% cross-linked agarose

The Co-NTA Resin is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6x His) tag expressed in bacteria, insects, and mammalian cells. The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. Although 6x His taggEd proteins bind with a lower efficiency compared to nickel chelating resins there is a significant reduction in non-specific binding.

The Co-NTA resin can be used to purify 6x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine. The Co-NTA resin uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand, in a highly cross-linked 6% agarose matrix. The NTA binds CO₂⁺ ions by four coordination sites.

We have demonstrated binding of >100 mg of a 50 kDa 6XHis tagged proteins to a ml of resin. The spin columns are supplied with a resin bed volume of 0.2, 1 and 3 ml with total column volumes of 1, 8 and 22 ml respectively. Columns can be used as a spin format of gravity flow columns. Also available in 96-well spin plate formats for processing up to 96 samples.

It is shipped at ambient temperature. Upon arrival, store it refrigerated at 4 °C, Do not freeze. This product is stable for 1 year at 4 °C.

Application: Affinity purification of proteins with a 6× His tag.