About this item
Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.
- High binding capacity: 38 mg human IgG/ml resin; >20 mg sheep IgG/ml resin
- Ligand: Recombinant Streptococcal protein A/G lacking the albumin binding sites expressed in E. coli
- Bead size: 50 to 165 μm
- Bead Structure: 6% highly cross-linked agarose
The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc.
Protein A/G binds well to IgG subclasses but does not bind IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and serum albumin. Individual subclasses of monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.
Storage conditions: It is shipped at ambient temperature. Upon arrival, store it refrigerated at 4 °C, Do not freeze. This product is stable for 1 year at 4 °C.
Immobilized Protein A/G is supplied as a 50% slurry in 20% ethanol/PBS solution. Suitable for immunoaffinity chromatography and immunoprecipitation.