Fast HiFi DNA Polymerase Master Mix is a ready-to-use 2X reaction mix composed of Fast HiFi DNA polymerase and an optimized buffer system, including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets.

• All-in-one 2X master mix for great convenience
• High fidelity: >60X Taq Fidelity
• 3’→5’ proofreading exonuclease activity
• Processes up to 11 kb from complex templates
• Good coverage on DNA templates with low to high GC content
• Fast extension rate: 10 s/kb

Fast HiFi DNA Polymerase 2X Master Mix is recommended for applications which require extremely high fidelity, such as cloning /subcloning, NGS applications and mutagenesis.

Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates.

The Fast HiFi DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine Enhancer Solution is recommended.

Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.