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SARS-CoV-2 Recombinant Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) (from HEK293 Cells)
SARS-CoV-2 Recombinant Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) (from HEK293 Cells)
Catalog # 76535-100
Supplier:  Sino Biological
CAS Number:  
SARS-CoV-2 Recombinant Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) (from HEK293 Cells)
Catalog # 76535-100
Supplier:  Sino Biological
Supplier Number:  40591V08H37100
CAS Number:  

Specifications

  • Conjugation:
    Unconjugated
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    SARS-CoV-2
  • Size:
    100 µg
  • Storage Conditions:
    –20…–80 °C
  • Endotoxin Content:
    <1.0 EU per μg protein as determined by the LAL method.
  • Biological Activity:
    Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag)(Cat:40591-V08H37), the EC50 is 12-80 ng/ml.
  • Gene ID:
    YP_009724390.1
  • Reconstitution Instructions:
    A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Carrier-Free:
    Y
  • Animal-Free:
    Y
  • Protein Synonyms:
    ncov RBD|ncov s2|ncov spike|coronavirus s2|S|s1|Spike RBD|ncov s1|coronavirus spike|coronavirus s1|cov spike|RBD
  • Protein/Peptide Name:
    Spike
  • Purity:
    >95% as determined by SDS-PAGE.
  • Molecular Weight:
    The recombinant SARS-CoV-2 Spike S1 consists of 679 amino acids and predicts a molecular mass of 76.06 kDa. As a result of glycosylation, it migrates as an approximately 114.84 kDa band in SDS-PAGE under reducing conditions.
  • Sequence:
    Met1-Arg685
  • Shipping Temperature:
    Ambient
  • Cat. No.:
    76535-100

Specifications

About this item

A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant C.1.2) which emerged in the South Africa.