SARS-CoV-2 Recombinant Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) (from HEK293 Cells)
Catalog # 76535-100
Supplier: Sino Biological
CAS Number:
Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:SARS-CoV-2
- Size:100 µg
- Storage Conditions:–20…–80 °C
- Endotoxin Content:<1.0 EU per μg protein as determined by the LAL method.
- Biological Activity:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag)(Cat:40591-V08H37), the EC50 is 12-80 ng/ml.
- Gene ID:YP_009724390.1
- Reconstitution Instructions:A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Carrier-Free:Y
- Animal-Free:Y
- Protein Synonyms:ncov RBD|ncov s2|ncov spike|coronavirus s2|S|s1|Spike RBD|ncov s1|coronavirus spike|coronavirus s1|cov spike|RBD
- Protein/Peptide Name:Spike
- Purity:>95% as determined by SDS-PAGE.
- Molecular Weight:The recombinant SARS-CoV-2 Spike S1 consists of 679 amino acids and predicts a molecular mass of 76.06 kDa. As a result of glycosylation, it migrates as an approximately 114.84 kDa band in SDS-PAGE under reducing conditions.
- Sequence:Met1-Arg685
- Shipping Temperature:Ambient
- Cat. No.:76535-100
Specifications
About this item
A DNA sequence encoding the SARS-CoV-2 Spike S1 (YP_009724390.1, with mutations P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant C.1.2) which emerged in the South Africa.