All-inclusive cell-based luciferase reporter assay kit targeting the Platelet-Derived Growth Factor Receptor alpha and beta, key receptors in atherosclerosis, pulmonary fibrosis, angiogenesis, and tumorigenesis research.

• Clear, reproducible results
• Freezer to data in 24 hours
• All-inclusive assay systems
• Exceptional cell viability post-thaw
• Consistent results lot to lot

INDIGO's Human Platelet-Derived Growth Factor Receptors α and β (PDGFRα/β) reporter assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to PDGFRα/β Reporter Cells, an optimized culture medium for use in reviving the cryopreserved cells, a culture medium for use in preparing test sample treatments, the reference physiological activator PDGF-BB, Luciferase Detection Reagents, and a cell culture-ready assay plate.

PDGFRα/β Reporter Cells are transiently transfected and prepared as frozen stocks using INDIGO's proprietary CryoMite™ process. This cryo-preservation method allows for the immediate dispensing of healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup.

INDIGO’s PDGFRα/β reporter assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.