About this item
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in variant of B.1.1.7 (Alpha strain), including: ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection
Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See the titration result showing the undiluted pseudovirus generates signal over 1000-fold higher than uninfected control (cell alone as background). The neutralizing activity of two control mAbs, including one published (Science, 2020: 369, p643) was tested by using this pseudotyped virus.
As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Applications:
•Anti-SARS-CoV-2 neutralizing antibody screening at high throughput
•Anti-SARS-CoV-2 drug screening at high throughput
•SARS-CoV-2 pseudovirus transduction of target cells for viral entry and functional studies
See suggested protocol to use this product.
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