Intact Genomics GV3101 chemically competent Agrobacterium cells are useful for Agrobacterium-mediated genetic transformation of Arabidopsis thaliana, tobacco, corn, potato, and other plants.
- High transformation efficiencies
- Especially useful for cDNA gDNA library construction
Intact Genomics GV3101 chemically competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.
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