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- Size:10U/µl
- Cat. No.:104013-272
Endonuclease IV (Nfo) from Escherichia coli is a 32 kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4).
- No detectable exonuclease or DNA N-glycosylase activity
- Useful for single cell gel electrophoresis, alkaline elution, and alkaline unwinding
Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radia-tion (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).
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